Peak distortion in reversed phase liquid chromatography (RPLC) as a consequence of viscosity differences between sample solvent and mobile phase

CASTELLS, C. B.; CASTELLS, R. C.

JOURNAL OF CHROMATOGRAPHY - A

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 1998 vol. 805 p. 55 - 61

0021-9673

Solute peaks may suffer severe distortions when their solutions in solvents whose viscosities differ from those of the mobile phase are injected in typical RPLC columns. A series of experiments with carefully chosen systems were performed, employing octyl silica columns, sample solvents whose viscosities were higher or lower than those of the corresponding mobile phases, and solutes that were more or less retained then the corresponding sample solvents. For a given viscosity difference, the importance of the distortions increases as the difference between solute and sample solvent retention times decreases. Chromatographic systems can be classified into four categories, and chromatograms illustrative of all of them were obtained.