Chiral high performance liquid chromatographic separation of -blockers by use of cellulose tris(3,5-dimethylphenylcarbamate) coated zirconia

C. B. CASTELLS; CARR, P. W.

JOURNAL OF CHROMATOGRAPHY - A

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2000 vol. 904 p. 17 - 33

0021-9673

In this work, we study the influence of the mobile phase and column temperature on the enantioresolution of basic compounds on microparticulate porous zirconia coated with cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC). The chiral analytes are amino compounds, including a number of b-blockers. Analytes are eluted with hexane?alcohol mobile phases.We investigated the effect of alcohol (type and concentration), basic eluent additives, and column temperature on the parameters that control resolution (column efficiency, retention and selectivity). Conditions for achieving an adequate separation in the least time have been determined for numerous racemic mixtures. For most solutes, baseline resolution of the enantiomeric pair was achieved in less than 1 min; 12 of 13 pairs were separated in less than 2 min.