INVESTIGADORES
BORSARELLI Claudio Dario
congresos y reuniones científicas
Título:
Towards efficient biocatalysts: photo-immobilization of a lipase on hybrid lysozyme-heparin amyloid nanofibrils
Autor/es:
S. CHAVEZ; C.M. ROMERO; R.A. MIGNONE; C. D. BORSARELLI; L. PERA; M. BAIGORI; R. CHEHIN
Lugar:
Sierra de la Ventana, BsAs
Reunión:
Congreso; XLIII REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE BIOFISICA; 2014
Institución organizadora:
SOCIEDAD ARGENTINA DE BIOFISICA
Resumen:
Amyloid fibrils have attracted nowadays a growing interest as new
biomaterials due to their special mechanical, chemical, and
structural properties, making them an excellent choice for
development of novel supports for different technological
applications. It is now widely accepted that the ability to form
amyloid aggregates is a common property of any polypeptide
chains. In fact, specific protocols for each protein have been
reported in order to turning them from soluble into highly ordered
amyloid aggregates with the characteristic cross- structures
among peptide chains. In the present work, we report the
preparation and characterization of a biocatalyst based on the
photo-immobilization of a lipase onto hybrid amyloid nanofibrils of
heparin and lysozyme. The new hybrid nanomaterial lost both the
lysozyme antibiotic activity and its ability to induce changes in
membrane permeability. However, the hybrid nanofibrils present
key reactive amino acids exposed to the solvent, such as tyrosine
residues, which allowed the covalent attach of a lipase molecule
through crosslinking via tyrosyl radicals generated by blue-light
photosensitization of the metal coordination complex ruthenium (II)
tris-bipyridine in the presence of ammonium persulfate. Thus, the
photo-immobilized lipase onto the hybrid nanofibrils showed much
better enzymatic activity under different extreme conditions of
temperature and solvent as compared with the free enzyme. The
procedure reported herein could be useful to design a new
generation of biocatalyst by a single photo-click step in a clean and
faster fashion way than conventional chemical crosslinked
procedure.