Development of a bioelectrode fabricated with a multilayer thin film of poly(diallyldimethylammonium)/gold-nanoparticle/lactate oxidase for analysis of L-lactate in food samples

PAZ ZANINI, VERÓNICA I.; LINAREZ PÉREZ, OMAR E.; TEIJELO, MANUEL LÓPEZ; LABBÉ, PIERRE; LOPEZ DE MISHIMA, BEATRIZ A.; BORSARELLI, CLAUDIO D.

SENSORS AND ACTUATORS B-CHEMICAL

ELSEVIER SCIENCE SA

Año: 2017 vol. 247 p. 830 - 839

0925-4005

Multilayer films made of poly(diallyldimethylammonium) (PDDA), gold nanoparticles (AuNP) of 10 nm diameter and lactate oxidase (LOx), were built for the first time, by layer-by-layer (LBL) assembly technique onto both quartz and gold substrates. The surface plasmon resonance (SPR) band of AuNP was used for monitoring the film growth and adsorption kinetics on PDDA monolayer supported onto quartz substrate. In the case of polycrystalline gold surfaces, electrochemical characterization was performed by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The latter system was used as bioelectrode for L-lactate quantification, showing a short time response (