Design of a new Electrogenerated Polyquinone Film Substituted with Glutathione. Towards Direct Electrochemical Biosensors

S. REISBERG; D.F. ACEVEDO; A. KOROWITCH; B. PIRO; V. NOEL; I. BUCHET; C.A. BARBERO; M.C. PHAM

TALANTA

ELSEVIER SCIENCE BV

Lugar: amsterdam; Año: 2010 vol. 80 p. 1318 - 1325

0039-9140

We developed a method to graft a tripeptide (glutathione) onto 5-hydroxy-1,4-naphthoquinone, an electropolymerizable molecule. The resulting thin conducting polymer presents a well-defined and stable electroactivity in neutral buffered solution, due to the embedded quinone group, and is able to covalently graft amino-modified DNA probe strands. It is shown that the bioelectrode presents positive current change following DNA hybridization. This makes a “signal-on” direct electrochemical DNA sensor. The results were obtained with low target concentration (50 nM) and the selectivity is excellent as a single-mismatch sequence can be discriminated from the full-complementary target