Histological changes of Berberis mikuna pollen grains in relation to viability and germinability

RADICE, SILVIA; GALATI, BEATRIZ G.; ZARLAVSKY, GABRIELA; ARENA, MIRIAM E

FLORA

ELSEVIER GMBH

Año: 2020

0367-2530

Berberis mikuna is a spontaneous shrub grown in the Tucuman-Bolivian subtropicaljungle area and its fruits were highly prized as food by diaguitas and calchaquies whoJournal Pre-proofwere local populations. Fruits are rich in phenolic compounds which would beresponsible for the beneficial properties for health. Pollen preservation in gene banksshould be implemented as standard procedures since pollen is a useful source of diversealleles within a gene pool. Therefore, the aim of this work was to study the histologicaland ultrastructural changes of pollen grains after 45 days of conservation at differenttemperatures. Berberis mikuna pollen grain germination and viability decreasedsignificantly after 45 days of its collection. Germination and viability decreased withincreasing storage temperature, although the viability in T45 Ref and T45 Fr was similar.Ultrastructure studies revealed differences in the fibrillar structure of the intine, as wellas the decrease in starch reserve in pollen grains stored at 5°C and at room temperature.