Polyamine endogenous variation and its incorporation in successive media for in vitro rooting of Nothofagus nervosa

G MARTINEZ PASTUR; M ARENA; MP BENAVIDEZ; E ELIASCO; N CURVETTO

NEW FORESTS

SPRINGER

Año: 2007 vol. 34 p. 83 - 93

0169-4286

An in vitro rooting protocol for producing Nothofagus nervosa microplants using successive steps during tissue culture is reported. Qualitative and quantitative changes in putrescine, spermidine, and spermine tissue contents, during the rooting process and their correlation with other biochemical markers and morphological changes, provided the rationale for their use during the study of a successive in vitro culture protocol for N. nervosa, as well as the use of their inhibitors. The polyamine tissue content was maximum before root emergence (spermine raised to 100 nmol/g FW to later decreased to ca. 50 nmol/g FW in the following days), but it was lower in improved rooting media (197 nmol/g FW compared to 264 nmol/g FW of the control medium). It was concluded that polyamines qualitatively and quantitatively improve in vitro rhizogenesis, and the best successive culture media included 10 ìM spermine during the expression phase.