INVESTIGADORES
ARENA Miriam Elizabeth
artículos
Título:
Polyamines and inhibitors used in successive culture media for in vitro rooting in Berberis buxifolia
Autor/es:
M ARENA; G MARTINEZ PASTUR; MP BENAVIDEZ; N CURVETTO
Revista:
NEW ZEALAND JOURNAL OF BOTANY
Editorial:
THE ROYAL SOCIETY OF NEW ZEALAND
Referencias:
Lugar: Wellington, Nueva Zelanda; Año: 2005 vol. 43 p. 373 - 380
ISSN:
0028-825X
Resumen:
Abstract Although Berberis buxifolia has been
reported to propagate in vitro, rooting is difficult to
achieve during subcultures, with reduced quantity
and quality of roots, as is the case with many other
woody species. Several cofactors of rhizogenesis
have been proposed, and the role of polyamines in
rooting has recently acquired relevance. Polyamines
can stimulate or inhibit microshoot rooting depending
on the type and concentration of polyamine
and rooting phase, while polyamine inhibitors can
improve rooting in some cases. A study of the in
vitro rooting of B. buxifolia using polyamines and
a mix of polyamine inhibitors in a two-step culture
medium is described here, in which a new successive
rooting medium was successfully implemented.
While polyamine inhibitors sometimes improved
rooting, nutrient medium containing a low polyamine
concentration (i.e., 1 µM) enhanced rooting
compared with the control medium. The best microshoot
rooting response resulted from the addition
of 1 µM spermidine during the expression phase
in the absence of inhibitors in both rooting phases.
Overall, incorporating polyamine in the successive
media enhances the quality and quantity of roots inAlthough Berberis buxifolia has been
reported to propagate in vitro, rooting is difficult to
achieve during subcultures, with reduced quantity
and quality of roots, as is the case with many other
woody species. Several cofactors of rhizogenesis
have been proposed, and the role of polyamines in
rooting has recently acquired relevance. Polyamines
can stimulate or inhibit microshoot rooting depending
on the type and concentration of polyamine
and rooting phase, while polyamine inhibitors can
improve rooting in some cases. A study of the in
vitro rooting of B. buxifolia using polyamines and
a mix of polyamine inhibitors in a two-step culture
medium is described here, in which a new successive
rooting medium was successfully implemented.
While polyamine inhibitors sometimes improved
rooting, nutrient medium containing a low polyamine
concentration (i.e., 1 µM) enhanced rooting
compared with the control medium. The best microshoot
rooting response resulted from the addition
of 1 µM spermidine during the expression phase
in the absence of inhibitors in both rooting phases.
Overall, incorporating polyamine in the successive
media enhances the quality and quantity of roots inin vitro, rooting is difficult to
achieve during subcultures, with reduced quantity
and quality of roots, as is the case with many other
woody species. Several cofactors of rhizogenesis
have been proposed, and the role of polyamines in
rooting has recently acquired relevance. Polyamines
can stimulate or inhibit microshoot rooting depending
on the type and concentration of polyamine
and rooting phase, while polyamine inhibitors can
improve rooting in some cases. A study of the in
vitro rooting of B. buxifolia using polyamines and
a mix of polyamine inhibitors in a two-step culture
medium is described here, in which a new successive
rooting medium was successfully implemented.
While polyamine inhibitors sometimes improved
rooting, nutrient medium containing a low polyamine
concentration (i.e., 1 µM) enhanced rooting
compared with the control medium. The best microshoot
rooting response resulted from the addition
of 1 µM spermidine during the expression phase
in the absence of inhibitors in both rooting phases.
Overall, incorporating polyamine in the successive
media enhances the quality and quantity of roots inin
vitro rooting of B. buxifolia using polyamines and
a mix of polyamine inhibitors in a two-step culture
medium is described here, in which a new successive
rooting medium was successfully implemented.
While polyamine inhibitors sometimes improved
rooting, nutrient medium containing a low polyamine
concentration (i.e., 1 µM) enhanced rooting
compared with the control medium. The best microshoot
rooting response resulted from the addition
of 1 µM spermidine during the expression phase
in the absence of inhibitors in both rooting phases.
Overall, incorporating polyamine in the successive
media enhances the quality and quantity of roots inrooting of B. buxifolia using polyamines and
a mix of polyamine inhibitors in a two-step culture
medium is described here, in which a new successive
rooting medium was successfully implemented.
While polyamine inhibitors sometimes improved
rooting, nutrient medium containing a low polyamine
concentration (i.e., 1 µM) enhanced rooting
compared with the control medium. The best microshoot
rooting response resulted from the addition
of 1 µM spermidine during the expression phase
in the absence of inhibitors in both rooting phases.
Overall, incorporating polyamine in the successive
media enhances the quality and quantity of roots inM) enhanced rooting
compared with the control medium. The best microshoot
rooting response resulted from the addition
of 1 µM spermidine during the expression phase
in the absence of inhibitors in both rooting phases.
Overall, incorporating polyamine in the successive
media enhances the quality and quantity of roots inM spermidine during the expression phase
in the absence of inhibitors in both rooting phases.
Overall, incorporating polyamine in the successive
media enhances the quality and quantity of roots in
B. buxifolia, thus confirming their role during the
induction and expression of the radicle primordia
differentiation., thus confirming their role during the
induction and expression of the radicle primordia
differentiation.