Skin immunity: IL-17 protects against epidermal dermatophytic infection and regulates the exacerbated inflammatory antifungal response.

BURSTEIN, VL; THEUMER, MG; GUASCONI, L; MENA, CJ; HERRERO, M; MASIH, DT; CHIAPELLO, LS

CABA

Congreso; I Meeting LASID-FAIC-SAI; 2015

Sociedad Argentina de Inmunología

Microsporum canis is a zoophilic dermatophytic that causes contagious skin infectionswith mild to severe inflammatory lesions, highly prevalent in immunocompetent children. Our previousdata demonstrate that epicutaneous infection of C57BL/6 mice with M. canis is characterized by a Th17response, however the in vivo role of IL-17 signaling has not been demonstrated. The aim of this study was to evaluate the impact of IL-17 in the outcome of skin infection and theantifungal inflammatory response.Wild type (WT) and IL-17RA-/- (KO) C57BL/6 mice were epicutaneously infected with M. canis and at 4,8, 18 and 45 days post-infection (d.p.i) histopathological analysis, skin fungal burden (HPLC ergosterolquantification) and extracutaneous fungal dissemination were determined. CD11b+ and T CD4+, TCD8+, Tγ� and B cell populations and its cytokine production were analyzed (ELISA or intracellularstaining and FACS) in skin cell suspensions or in skin draining lymph nodes cells (sdLN) after antigenspecific-reestimulation. In addition, survival rate was registered.WT and KO mice resolved infection by 18 d.p.i., however KO mice showed severe inflammatoryresponse in skin and sdLNs (p