Systemic infection with Candida albicans induces Dectin-1 expression in brain

VIGEZZI C; MIRÓ MS; ICELY PA; RODRIGUEZ EMILSE; CEJAS H; SOTOMAYOR CE

Mar del Plata

Congreso; LIX Reunion anual de la Sociedad Argentina de Investigación Clínica y LXII reunión científica anual de la Sociedad Argentina de Inmunología,; 2014

Sociedad Argentina de Investigación Clínica y LXII reunión científica anual de la Sociedad Argentina de Inmunología

Candida albicans is an opportunistic fungus that can cause brain infection in infants and immunocompromised host. Recognition of wall structures by C.albicans glucan receptor Dectin-1 is essential for the antifungal response in the periphery. At present little is known about the expression of this receptor in the Central Nervous System (CNS). Our objective was to evaluate the expression of Dectin-1 in the brain during the course of disseminated infection by this fungus. Male C57BL/6 mice were injected iv with 2,5.106 viable yeasts (ATCC36801) and at 12, 24, 48 and 72h post-infection (pi), animals were weighted to determine the Index of Body Weight (IBW) and sacrificed to obtain kidney, liver and brain to different studies: organ weight, fungal burden (CFU), histopathological changes (HE) and Dectin-1 expression (WB). The infection caused a significant decrease in the IBW of infected animals that was observed from 12h pi (p