Frequency, virulence factors and antifungal susceptibility of Candida parapsilosis species complex isolated from patients with candidemia in the central region of Argentina

VIGEZZI C; RODRIGUEZ E; AZCURRA AI; RIERA FO; VIGEZZI C; ICELY PA; RODRIGUEZ E; MIRÓ MS; AZCURRA AI; ABIEGA C; RIERA FO; GARCÍA-EFFRON G; ICELY PA; MIRÓ MS; ABIEGA C; GARCÍA-EFFRON G; DUDIUK C; CASTILLO GDV; CAEIRO JP; SOTOMAYOR CE; DUDIUK C; CASTILLO GDV; CAEIRO JP; SOTOMAYOR CE

JOURNAL DE MYCOLOGIE MEDICALE

MASSON EDITEUR

Lugar: Paris; Año: 2019

1156-5233

Purpose. ? Our objectives were to report species distribution and survival of patients with candidemia in Argentina?s central region and to establish the prevalence of C.parapsilosis sensu lato species, their virulence factors and their antifungal susceptibility profiles.Methods. ? Yeasts isolated from bloodstream infections in Co´ rdoba (Argentina) (n = 35) were molecularly identified. The production of lipase and acid aspartic protease (Sap), the adhesion capacity, and the isolates? ability to form biofilm were evaluated. The in vitro activity of 7 antifungal drugs wasevaluated (CLSIdocument M27-4thed).Results. ? C. albicans was the most prevalent species (48.57%) followed by C. parapsilosis sensu lato(28.57%). The 30-day survival rate for C. albicans candidemia was slightly lower than non-albicans blood infections (50.00% vs. 57.90%). C. parapsilosis sensu stricto and C. orthopsilosis account for 60% and 40% of the cryptic species. Sap production and biofilm formation capacity were higher in C. parapsilosis sensu strico than in C.orthopsilosis. All the strains were susceptible to caspofungin (CAS), anidulafungin (AFG), amphotericin B (AMB), posaconazole (POS) and voriconazole (VRC). Azoles were the most potent agent against C. parapsilosis sensu lato followed by echinocandins and AMB. There were no differences between MICs for fluconazole, VRC, POS and AMB. Contrarily, C. parapsilosis sensu stricto strains showed lower MICthan C. orthopsilopsis isolates for itraconazole and higher MIC values for echinocandins (P < 0.01).Conclusions. ? We report a high frequency of isolation of C.orthopsilosis in candidemia patients of centralregion. Data on the prevalence, virulence capability and antifungal susceptibility of C. parapsilosis complex provide new epidemiological information about these cryptic species in Argentina.