MTs in Palaemonetes argentinus as potential biomarkers of zinc contamination in freshwaters

BERTRAND, LIDWINA ; MONFERRÁN, MAGDALENA VICTORIA ; MÉTAIS, ISABELLE ; MOUNEYRAC, CATHERINE; AMÉ, MARÍA VALERIA

ECOLOGICAL INDICATORS

ELSEVIER SCIENCE BV

Lugar: Kiel; Año: 2014 vol. 48 p. 533 - 541

1470-160X

Aquatic invertebrates take up and accumulate essential and non-essential trace metals even when both are likely to be poisonous. In order to study the potential of the metallothioneins (MTs) as biomarkers of metal contamination in native shrimp Palaemonetes argentinus, organisms have been exposed at 0, 5, 50 and 500 mg L-1 of zinc for 96 h. Moreover, accumulation and subcellular distribution of this essential metal were evaluated. A significant Zn accumulation was observed in different body sections. Higher Zn levels occurred in cephalothorax compared to abdomen, especially at the highest exposure concentration (500 mg Zn L-1). A clear differential subcellular metal distribution between cephalothorax and abdomen was also observed. In cephalothorax Zn was similarly distributed between the soluble and insoluble fractions; while in abdomen, when total Zn increased, insoluble metal augmented more markedly than the soluble one. Cytosolic Zn levels increased more in cephalothorax than in abdomen of shrimps exposed to 500 mg Zn L-1 when compared to control. Finally, a significant induction of MTs was observed in cephalothorax at 500 mg Zn L-1. A potential role for MTs as biomarkers in P. argentinus should be further studied to enhance the sensitivity of the response, although it is likely that MTs play a key role in metal detoxification since the increase of these proteins is linked to metal challenge.