Plasmid diversity in an A. bereziniae HPC229 clinical strain: antimicrobial and heavy metal resistance genes, different replicases, and XerC/D-flanked modules contribute to the adaptability to different habitats and to the Acinetobacter plasmidome

BROVEDAN M; LIMANSKY A; VIALE AM; MARCHIARO P; REPIZO GD

Conferencia; Plasmid Biology 2018; 2018

Acinetobacter bereziniae (Abe) is primarily isolated from clinical specimens and health care associated environments. Although most Abe isolates aresusceptible to antimicrobials, clinical strains bearing metallo-β-lactamase genes have just been described. We have recently reported the whole-genomesequencing (WGS) of the β-lactam resistant Abe strain HPC229, thus constituting the sixth Abe genome deposited in public databases (1). We also described thatthis strain carries a bla -bearing plasmid designated pNDM229 (2), which sequence is similar to that of the conjugative plasmid pNDM-BJ01 from an A. lwofi NDM-1strain isolated in China (3). We characterized here the whole HPC229 plasmidic content, and conducted a comparative analysis against the Acinetobacter sppdatabase. Also, plasmids were classied on the basis of its replication machinery, employing the A. baumannii plasmid-borne replicase proteins including allrepresentative Rep groups (GR; 4).