IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Novel bromodomain containing proteins pattern expression in Trypanosoma cruzi
Autor/es:
VILLANOVA, V; BARISON, MJ; ALONSO, V; TROCHINE A; MAGDALENO, A; SILBER, A; SERRA ESTEBAN; CRIBB, P
Lugar:
Santa Fe
Reunión:
Congreso; XXIII Reunión Anual. Sociedad Argentina de Protozoología; 2009
Institución organizadora:
Sociedad Argentina de Protozoología
Resumen:
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TcBDF1-5 are bromodomain containing
factors (BDF) codified in Trypanosoma
cruzi genome. Bromodomain is a highly conserved domain in eukaryotes that
binds acetyl-lysines. All BDFs reported up to now in other species, show
nuclear localization and are associated to other domains or enzymatic
activities taking part of multisubunit complexes.
TcBDF-1 and -3 expression in T. cruzi was analyzed by western blot
(WB) and immunofluorescence (IFI) using purified antibodies. TcBDF1 expression
was observed in epimastigotes by WB in the cytoplasmatic fraction. A spotted
pattern was seen in the posterior part of epimastigotes and along all the
parasite body in trypomastigotes by IFI. TcBDF2 is a nuclear protein in all
life cycle stages and it interacts with acetylated histones.TcBDF3 expression
was observed in all life cycle stages by WB. It was seen in epimastigotes and
amastigotes cytoplasm and at flagella in trypomastigotes and metacyclic trypomastigotes.
Surprisingly both TcBDF1 and 3 are not nuclear proteins. We are analyzing their
relationship with acetylated proteins in cytoplasm and flagella.TcBDF5 has two tandemly
arranged bromodomains. It is bigger in size than the other BDFs. We cloned,
expressed and purified its bromodomain region as histidine fusion and used it
to obtain antibodies to analyze its cellular localization.
It has been proposed that protein
acetylation could act as a regulatory modification and that bromodomains may
act recognizing acetyl-lysine and transducing the signal, like phosphorilation
and SH2 domains do. The broad TcBDFs pattern expression presented here suggests
that this regulatory mechanism could be present in T. cruzi.