IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Finding molecular targets for the nucleic acid chaperone CNBP.
Autor/es:
ARMAS, P.; RUZZI, L.R.; CALCATERRA, N.B.
Lugar:
San Miguel de Tucumán, Tucumán, Argentina.
Reunión:
Congreso; XLV Annual Meeting of the Argentine Society for Biochemestry and Molecular Biology Research.; 2009
Institución organizadora:
Argentine Society for Biochemestry and Molecular Biology Research.
Resumen:
Cellular nucleic acid binding protein (CNBP) is a conserved
single-stranded nucleic acid binding protein required for vertebrates
craniofacial development. CNBP acts as a nucleic acid chaperone,
rearranging the secondary structure of its targets. Recently, our group
demonstrated that CNBP promotes the formation of G-quadruplexes,
stable nucleic acid structures described as novel elements for gene
expression regulation. However, the consensus sequence of CNBP targets
has not been elucidated yet. The main goal of this work was to gain
knowledge about the common features among CNBP molecular targets in
order to identify putative genes regulated by this protein. Therefore,
we performed a Systematic Evolution of Ligands
by Exponential Enrichment (SELEX) using zebrafish CNBP and a single-stranded nucleic acid
library degenerated in 16 nucleotides. Sequences obtained with this
procedure have been analyzed and compared with previous reported
targets in order to establish a consensus sequence. On the other hand,
we performed a genome-wide screening for zebrafish CNBP binding sites
using inverse monohybrid strategy on a genomic zebrafish library
cloned in yeast. The screening provided the identity of putative target
genes that may be controlled by CNBP. These results will help to better understand how CNBP performs
its essential biological function in embryonic development.