CONTROL OF PLANT DEVELOPMENT BY THETRANSCRIPTIONAL COACTIVATOR AN3

ERCOLI, MA FLORENCIA; PALATNIK, JAVIER F; PERRONE, ANA PAULA; RODRIGUEZ, RAMIRO ESTEBAN; DEBERNARDI, JUAN MANUEL

Córdoba

Congreso; 52th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2016

Argentine Society for Biochemistry and Molecular Biology

In Arabidopsis thaliana, GRF-INTERACTING FACTORS (GIFs) gene family consists of AtGIF1, also known as ANGUSTIFOLIA3 (AN3), AtGIF2 and AtGIF3. They have homology to human SYNOVIAL TRANSLOCATOR transcriptional coactivator and have been identified as transcriptional coactivators. GIFsproteins interact with the GROWTH REGULATING FACTORS transcription factors and BRAHMA, a central ATPase component of the chromatin remodeling complex. The AN3gene has a central role in the control of cell proliferation and therefore in plant size. AN3 mutants have lanceolated leavesand a modified meristematic activity. AN3 is a small protein that can move from cell to cell through plasmodesmata. Here, we studied the importance of AN3 movement in different biological processes. To do this, we prepared translational fusions of AN3 genomic sequence (gAN3) to single (gAN3GFP) or triple molecules of GFP (gAN33XGFP).The later chimeric protein cannot move from cell to cell due to its larger size. We expressed these proteins from their own regulatory sequences as well as tissue specific promoters. We analyzed the ability of AN3 to move across different cells and to complement AN3 mutants. The results provide new insights about the control of AN3 expression and the role of AN3 movement during development.