Purine metabolism and polyketide production in Streptomyces coelicolor

LAURA NAVONE, HUGO GRAMAJO, AND EDUARDO RODRIGUEZ

Potrero de Funes

Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigadores en Bioquímica y Biología Molecular (SAIB).; 2011

Bacteria from the genera Streptomyces produce a vast array of secondary metabolites of industrial and medical interest. Among these metabolites, polyketides compounds are especially important due to the diversity of biological activities they comprise (insecticides, inmunosupresors, antibiotics, anticancerous). These natural compounds are synthesized by a multienzymatic complex under certain conditions of growth and are regulated by specific transcriptional regulators. In turn, these transcriptional factors respond to intra and extracellular signals sensed by a set of global regulators. We have previously identified a gene in S. coelicolor, allR, encoding a transcriptional regulator that strongly impairs antibiotic production when inactivated. AllR negatively controls the expression of genes required for allantoin catabolism. This metabolic pathway is involved in the recycling of the carbon and nitrogen components of purines generated during nucleotide turnover. Proteome analysis of allR mutant strain has shown increase expression of proteins involved in the tricarboxylic acid cycle, biosynthesis of aminoacids and metabolism of purines among others. This result demonstrates that AllR is involved in the expression of other metabolic pathways and represents an important link