LIPID METABOLISM REGULATION BY THE PROINFLAMMATORY CYTOKINE TNF

FRANCISCO DAMIÁN ROSA; MILENI SOARES MACHADO; NATALIA PALADINO; IGNACIO AIELLO; GABRIELA INÉS MARINO; MARÍA FERNANDA RUBIO; MARÍA CECILIA LIRA; ALEJANDRA G. PALMA; MÓNICA A COSTAS

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencia 2019; 2019

Sociedad Argentina de Investigación Clínica

The crosstalk between the cancer cells and those from the tumor microenvironment are important in cancer biology. In breast cancer, there is a deregulated secretion of cytokines and chemokines from the adipose tissue (AT) that can contribute to tumor onset and progression. In a previous work, we have seen that the human AT surrounding breast cancer has a greater TNF expression compared to AT adjacent to normal mammary. Since TNF can induce adipocyte lipolysis and, hence, support the anabolic tumor metabolism, we measured triglyceride secretion in murine mammary gland AT conditioned media (C57BL-6j) after 48 h with or without TNF 10 ng/ml. We found that TNF increased the triglyceride secretion (5.8 g/l vs. 0.93 g/l basal; p0.01). We also performed a total extraction of lipids from the tissue of all the above conditions. We observed a decreased in the total content of lipids only under TNF stimulus (TNF: 0.77 respect to basal, p