THE EXPRESSION LEVELS OF RAC3 COACTIVATOR IN THE BREAST ADIPOSE TISSUE ARE ALTERED IN THE TUMORAL CONTEXT

ROSA, FRANCISCO D; SALAZAR, CECILIA; LIRA, MARÍA CECILIA; PANELO, LAURA C; RUBIO, MARÍA FERNANDA; SOARES MACHADO, MILENI; COSTAS, MÓNICA A

CABA

Congreso; LXII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2017

Sociedad Argentina de Investigación Clínica

Several types of tumors grow in the adipose tissue anatomical neighborhood. It is well known that there is a communication between tumors and adipocytes, which are around them, that involves the secretion of different factors.RAC3 coactivator is over expressed in a broad variety of cancers, including breast cancer. Previously, in the L-929 fibroblastic murine line, we demonstrate that the decrease of RAC3, using an interference RNA, favors the adipogenesis through an increase of autophagy and a decrease in the proliferative rate.In this work, we aimed to determine RAC3 expression levels in L-929 cells during the differentiation. The transcript levels of RAC3 were characterized by qPCR at different times after induction with 0.1 g/ml insulin, 1 M dexamethasone, 0.1 mM indomethacin and 0.5 mM 3-isobutyl-1-methylxanthine (DI3).Notably, the levels of RAC3 mRNA decrease in the studied temporal range (1.0 ± 0.1 at 0h; 0.3 ± 0.1 at 30h; 0.2 ± 0.1 at 48h, p