Oxidative stress induces arginine kinase expression in Trypanosoma cruzi.

MIRANDA, MARIANA; CANEPA, GASPAR; BOUVIER, LEON; PEREIRA, CA

EXPERIMENTAL PARASITOLOGY

Elsevier

Año: 2006 vol. 114 p. 341 - 344

0014-4894

Trypanosoma cruzi arginine kinase is a key enzyme in cell energy management and is also involved in pH and nutritional stress response mechanisms. T. cruzi epimastigotes treated with hydrogen peroxide presented a time-dependent increase in arginine kinase expression, up to 10-fold, when compared with untreated parasites. Among other oxidative stress-generating compounds tested, only nifurtimox produced more than 2-fold increase in arginine kinase expression. Moreover, parasites overexpressing arginine kinase showed signiWcantly increased survival capability during hydrogen peroxide exposure. These Wndings suggest the participation of arginine kinase in oxidative stress response systems.arginine kinase is a key enzyme in cell energy management and is also involved in pH and nutritional stress response mechanisms. T. cruzi epimastigotes treated with hydrogen peroxide presented a time-dependent increase in arginine kinase expression, up to 10-fold, when compared with untreated parasites. Among other oxidative stress-generating compounds tested, only nifurtimox produced more than 2-fold increase in arginine kinase expression. Moreover, parasites overexpressing arginine kinase showed signiWcantly increased survival capability during hydrogen peroxide exposure. These Wndings suggest the participation of arginine kinase in oxidative stress response systems.T. cruzi epimastigotes treated with hydrogen peroxide presented a time-dependent increase in arginine kinase expression, up to 10-fold, when compared with untreated parasites. Among other oxidative stress-generating compounds tested, only nifurtimox produced more than 2-fold increase in arginine kinase expression. Moreover, parasites overexpressing arginine kinase showed signiWcantly increased survival capability during hydrogen peroxide exposure. These Wndings suggest the participation of arginine kinase in oxidative stress response systems.Wcantly increased survival capability during hydrogen peroxide exposure. These Wndings suggest the participation of arginine kinase in oxidative stress response systems.