IDIM 12530
INSTITUTO DE INVESTIGACIONES MEDICAS
Unidad Ejecutora - UE
artículos
Título:
Trypanosoma cruzi amino acid transporter TcAAAP411 mediates arginine uptake in yeasts
Autor/es:
CARRILLO, CAROLINA; CANEPA, GASPAR; GIACOMETTI, ALINA; BOUVIER, LEON; MIRANDA, MARIANA; CAMARA, MARIA DE LOS MILAGROS; PEREIRA, CA
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
Elsevier
Referencias:
Año: 2010 vol. 306 p. 97 - 102
ISSN:
0378-1097
Resumen:
Trypanosoma cruzi, the aetiological agent of Chagas disease, is exposed toextremely different environment conditions during its life cycle, and transportersare key molecules for its adaptive regulation. Amino acids, and particularlyarginine, are essential components in T. cruzi metabolism. In this work, a novelT. cruzi arginine permease was identified by screening different members of theAAAP family (amino acid/auxin permeases) in yeast complementation assaysusing a toxic arginine analogue. One gene candidate, TcAAAP411, was characterizedas a very specific, high-affinity, L-arginine permease. This work is the firstidentification of the molecular components involved specifically in amino acidtransport in T. cruzi and provides new insights for further validation of theTcAAAP family as functional permeases.
