CIHIDECAR   12529
CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO
Unidad Ejecutora - UE
capítulos de libros
Título:
Nanoparticles Applied to Mass Spectrometry Metabolomics and Pesticide Residue Analysis
Autor/es:
YOUSEF GHOLIPOUR; ROSA ERRA BALSELLS; HIROSHI NONAMI
Libro:
Nanotechnology and Plant Sciences
Editorial:
Springer International Publishing
Referencias:
Lugar: Ginebra; Año: 2015; p. 289 - 303
Resumen:
Abstract In this chapter, we review the achievements in application of nanoparticles
and nanotubes to mass spectrometry metabolomics of plants. Ultraviolet
matrix-assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS)
using nanoparticles as the matrices enables biologists to directly analyze metabolite
composition of plant tissues. In addition, due to its high sensitivity and low
limit of detection, it is applied to plant single-cell analysis for metabolite profiling
of pico- to nanoliter of cell microsample with metabolite concentration of
femto- to picomolar. In addition to the detection, the technique provides quantitative
information of metabolite composition of plant single cells. Another interesting
feature of nanoparticle UV-MADI-MS is its applicability to direct analysis of
pesticide residues on agricultural products. The technique has been able to detect
main component and degradation products of commercial pesticides sprayed on
plants in a greenhouse.