INQUIMAE 12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Chromophore heterogeneity and photoconversion in phytochrome crystals and solutions studied by Resonance Raman spectroscopy
Autor/es:
VON STETTEN, D.; GÜNTHER, M.; SCHEERER, P.; MURGIDA, D. H.; MROGINSKI, M. A.; KRAUß, N.; LAMPARTER, T.; ZHANG, J.; ANSTROM, D. M.; VIERSTRA, R. D.; FOREST, K. T.; HILDEBRANDT, P.
Revista:
Angewandte Chemie
Referencias:
Año: 2008 vol. 47 p. 4753 - 4755
ISSN:
1433-7851
Resumen:
Crystals of the photoreceptor phytochrome were studied
nondestructively by resonance Raman spectroscopy. The spectra of the
chromophore-binding domains (CBD) of phytochrome from Deinococcus radiodurans,
from which three-dimensional structures have been determined,
demonstrate the same chromophore structure in the parent Pr state as in
solution. Unlike CBD, crystals of phytochrome from Agrobacterium tumefaciens including the CBD and the PHY domain can be photoconverted to a Meta-Rc-like state.
