INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Absorption and Scattering Coefficients. A Biophysical-chemistry experiment using Reflectance Spectroscopy
Autor/es:
GABRIELA B. CORDON AND M. GABRIELA LAGORIO
Revista:
JOURNAL OF CHEMICAL EDUCATION
Editorial:
American Chemical Society
Referencias:
Lugar: Wisconsin; Año: 2006
ISSN:
0021-9584
Resumen:
Abstract The scattering (S) and the absorption (K) coefficients of leaves from different plant species were determined separately as a function of wavelength from reflectance and transmittance data measured for individual leaves. The equations that link the measurable data with the optical parameters K and S, were deduced using both Kubelka-Munk approach and the model of the ¨ Pile of plates ¨. In parallel, the reflectance of an optically thick sample of stacked leaves were measured and the remission function was calculated. The values of the remission function obtained at different wavelengths from stacked leaves were compared with the quotients of the optical parameters K/S, obtained from individual leaves. Both values are predicted to be equal according the Kubelka-Munk theory. The accomplishment of this equality shows that the pile of plates model is a good description for the optical properties of leaves. The scattering (S) and the absorption (K) coefficients of leaves from different plant species were determined separately as a function of wavelength from reflectance and transmittance data measured for individual leaves. The equations that link the measurable data with the optical parameters K and S, were deduced using both Kubelka-Munk approach and the model of the ¨ Pile of plates ¨. In parallel, the reflectance of an optically thick sample of stacked leaves were measured and the remission function was calculated. The values of the remission function obtained at different wavelengths from stacked leaves were compared with the quotients of the optical parameters K/S, obtained from individual leaves. Both values are predicted to be equal according the Kubelka-Munk theory. The accomplishment of this equality shows that the pile of plates model is a good description for the optical properties of leaves.