Substances induced changes in mobility of the organism C. elegans in microfluidic device

HUILCA, G.; PEREZ, M.; KRONBERG, M. FLORENCIA; MAIER,D.

Cordoba

Congreso; II Brazil-Argentine Microfluidics Congress; 2019

Microfluidics is based on the study of several applications with different organisms,this is the case of lab on a chip. (LOC). (Bourguignon, 2018). The use ofthis microdevice is given in eukaryotic and prokaryotic cells and in some nematodessuch as Caenorhabditis elegans. The worm is useful in the study of neurons,genes, mobility, behavior, sometimes it has been complicated to control the chemicalstimuli, which behave randomly in each of the larval stages. The worm isimportant for the study of mechanosensation and chemosensation. Its nervoussystem contains 302 neurons, its genome is completely sequenced. There are 6receptors that identify external factors, they are found in the cuticle of the body.It also contains 95 muscle cells. (Johari. 2013). The mobility of the worm can beidentified according to the time taken by C elegans from the entrance to the exitof the chip. The device that was used in the process is E10, it has 4 channels, oneinput and one output at each end. It is designed with the compound polymethylmethacrylate(PMMA) and polydimethylsiloxane (PDMS). In one of the outlets,strain E coli was placed, with an optimum density of 0.45. The larval stage ofthe nematode that was placed in the device was L1, L3, L4 and adult. The wormsin the adult stage, have a length of 1 mm and a diameter of 60 m, is the rightsize for insertion into the chip. C elegans, is used in many applications, highlightthe phenotypic screens, images of neurons, taxonomy with the laser beam. In thepresent investigation, it is recommended the use of a new microfluidic system,consisting of an enter channel and an exit, and several obstacles inside the chip,so that C elegans can adhere to the device. This chip allows the worm to move ina matrix. Finally, an analysis of mobility was carried out, with respect to threesubstances that change behavior. It was found that the olfactory behavior is givenaccording to the locomotion and the need to look for food, as well as the differentcompounds that are used. (Albrecht, 2012).