Plant Cell Growth and Expansion. Series Methods in Molecular Biology.

DENITA JUAREZ, S. MANGANO & J.M. ESTEVEZ.

Springer Science+Business Media New York

Año: 2015 p. 5

978-1-62703-579-8

Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth(1). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2´, 7-dihidro dicloro fluorescein (H2DCFDA).