Platelets bioenergetics screening reflects the impact of brain Abeta plaque accumulation in a rat model of Alzheimer

GALEANO, PABLO; CASTAÑO, EDUARDO MIGUEL; PRESTIA, FEDERICO ARIEL; DO CARMO, SONIA; MORELLI, LAURA; MARTINO ADAMI, PAMELA VICTORIA; CUELLO, AUGUSTO CLAUDIO

Córdoba

Congreso; Congreso de la Sociedad Argentina de Investigación en Neurociencias, Edición 2018; 2018

Sociedad Argentina de Investigación en Neurociencias

Alzheimer disease (AD) is associated to depressed brain energy supply and impaired cortical and hippocampal synaptic function. It was previously reported in McGill-R-Thy1-APP transgenic (Tg(+/+)) rats that Abeta deposition per se is sufficient to cause abnormalities in glucose metabolism and neuronal connectivity. These data support the utility of this animal model as a platform for the search of novel AD biomarkers based on bioenergetic status. Recently, it has been proposed that energy dysfunction can be dynamically tested in platelets (PLTs) of nonhuman primates. PLTs are good candidates to find peripheral biomarkers for AD because they may reflect in periphery the bioenergetics deficits and the inflammatory and oxidative stress processes taking place in AD brain. In the present study, we carried out a PLTs bioenergetic screening in advanced-age (12-14 months old) control (WT) and Tg(+/+) rats and found a significant decrements in the maximal respiration of thrombin-activated PLTs of Tg(+/+) as compared to WT rats. In summary, our results provide original evidence that PLTs bioenergetic profiling may reflect brain bioenergetics dysfunction mediated by Abeta plaque accumulation. Further studies on human PLTs from control and AD patients are required to validate the usefulness of PLTs bioenergetics as a novel blood-based biomarker for AD.