Platelets bioenergetics as a potential biomarker for Alzheimer?s disease.

MARTINO ADAMI PV; CASTAÑO, E.M.; PRESTIA, F.A.; CUELLO, C; GALEANO P; MORELLI L

Ciudad Autónoma de Buenos Aires

Congreso; Reunion de Sociedades Conjuntas; 2017

Sociedad Argentina de Investigación Clínica (SAIC)

Bioenergetic dysfunction has been suggested as an early eventand as a cause for synaptic deficiency and cognitive impairment inAlzheimer disease (AD). It has been proposed that energy dysfunctioncan be dynamically tested in Platelets (PLTs). PLTs are goodcandidates to find peripheral biomarkers for AD because may reflectin periphery the inflammatory and oxidative stress that happens inthe brain. We aimed to perform a time-course analysis of PLTs isolatedfrom peripheral blood of control (CNT) and hemizygous McGill-R-Thy1-APP transgenic (Tg+/-) rats, a well characterized model ofearly AD. We used an extracelular flux analyzer (Seahorse XFp) and2 different approaches: MitoStress (evaluates mitochondria functionality)and GlycoStress (asseses glycolitic performance). Animals (n= 3-6/group) from 3-12 month-old were anestethized, beheaded and blood (10 ml) recovered in sterile tubes containing Citrate Buffer/Dextrose (pH 7.4). PLTs were isolated by sequential centrifugation at room temperatura to avoid activation. Quantification was performed by hematologic analyzer (Mindray 5800). 15x106 PLTs/well were seeded in XFp Cell Culture MiniPlates and centrifuged (1500g/15 min), afterthat supernatant was removed and MitoStress and GlycoStress performed. We set-up a method to evaluate mitochondrial activity in PLTs and are able to plot profiles to compare CNT vs. Tg(+/-). By contrast to synaptosomes, PLTs do not have spare respiratory capacity, it means that are not involved in process