Lipid-laden dendritic cells in protective melanoma immunotherapy

MAC KEON SOLEDAD; BERGUER PAULA; MORDOH JOSÉ; WAINSTOK ROSA

Tours

Simposio; 13th International Symposium on Dendritic Cells; 2014

Development and optimization of immunotherapeutic therapies hold a great promise in the field of melanoma treatment. Recently it has been shown that in dendritic cells (DC), cells in charge of the acquisition, processing and presentation of tumor antigens to T cells, lipid accumulation in cancer settings impairs their correct functionality. Nonetheless, the question of how DC neutral lipid content affects their immunological role in the development of anti-tumoral responses remains unresolved. In this work we show in a melanoma immunotherapeutic experimental setting how DC co-cultured with apoptotic and necrotic B16-F1 melanoma cells (Apo/Nec) accumulate large-sized lipid droplets (LD). This lipid-laden DC, although being poorly stimulated by maturation cocktails in vitro, are capable of inducing lymph-node tumor-specific CD8+ responses and a potent anti-melanoma protection. This work contributes to the understanding of the immunological role of lipid-laden DC in cancer immunotherapy.