Transcriptome analyses identifies specific intracellular pathways and target genes in gastric cancer

BIZAMA, C.; BENAVENTE, F.; ESPINOZA, JA.; SALVATIERRA, E.; FERNANDEZ, EA.; SAGREDO, E; ROA, I.; MAZZOLINI G; GIDEKEL, M.; PODHAJCER, O. L.

Puerto varas

Congreso; XXXV Reunion Anual Sociedad de Bioquimica y Biologia Molecular de Chile.; 2012

Sociedad de Bioquimica y Biologia Molecular de Chile.

Studies on the low abundance transcriptome are of paramount importance to identify the intimate mechanisms of tumor progression. By using subtractive hybridization followed by transcriptome analysis of human samples obtained from gastric and colon cancer and paired adjacent non-malignant tissues we identified novel cancer biomarkers such as LAMA3 and TTN, highly specific intracellular pathways and gene targets such as IRS2, IL17, IFNã, VEGF-C, WISP1, FZD5 and CTBP1 that were not detectable by direct transcriptomic analyses. Knocking down the expression of CTBP1 sensitized gastric cancer cells to 5-FU, cisplatin and epirubicin that are part of the mainstay repertoire for gastric cancer treatment. The use of these affordable combined platforms might have important implications in the understanding and treatment of cancer.