Synthetic tumor specific promoters for transcriptional regulation of viral replication

LOPEZ MARIA VERONICA; PODHAJCER OSVALDO L; VIALE DIEGO L; CAFFERATA EDUARDO G

Methods in Molecular Biology: Mammalian Synthetic Promoters

Springer International Publishing AG.

Año: 2017; p. 113 - 130

Synthetic tumor specific promoters for transcriptionalregulation of viral replication. M. Veronica Lopez, Eduardo. G. Cafferata, Diego. L. Viale and OsvaldoPodhajcer SummaryHere we describe a collection of methods that havebeen adapted to isolate and modify tumor specific promoters (TSP) to driveviral replication for cancer therapy and other uses. We will describe asexamples the secreted protein acidic and rich in cysteine (SPARC) and the protease-activatedreceptor-1 (PAR-1) promoter. We outline strategies to select appropriate TSPusing bioinformatics resources and the methods utilised in their subsequentcloning, assessment of transcriptional activity and their use in conditionally replicative oncolyticadenoviruses. Key Words: synthetic tumor specific promoter; adenovirus;