Application of Synthetic tumor specific promoters responsive to the tumor microenvironment.

LOPEZ MARIA VERONICA; PODHAJCER OSVALDO L; CAFFERATA EDUARDO G; RUSSO MAENZA MARIA AGOSTINA ; NUÑEZ FELIPE

Methods in Molecular Biology: Mammalian Synthetic Promoters

Springer International Publishing AG.

Año: 2017; p. 213 - 227

Application of Synthetic tumor specificpromoters responsive to the tumor microenvironment.Eduardo G Cafferata, M Verónica López, Felipe J Nuñez,María Agostina Russo Maenza and Osvaldo L Podhajcer.Summary/Abstract Activity of endogenous promoters can be altered byincluding additional responsive elements (RE). These elements can be responsiveto features of the tumor environment or alternatively to signaling pathwaysspecifically activated in cancer cells. These REs incorporated into tumorspecific promoters can improve cancer targeting, the replicative capacity andlytic activity of conditionally replicative adenovirus. Here we outline an approach to incorporate hypoxia andinflammation REs into a specific fragment of the SPARC promoter and the steps to clone a nucleosome positioning sequence (NPS) identifiedin the osteocalcin promoter that contains a Wnt RE upstream of a heterologoussynthetic promoter. Key Words Synthetic tumor specific promoter; hypoxia responsive element, nuclear factor kappa B -responsive element, Wnt-responsive element, nucleosomepositioning sequence,adenovirus.