IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
artículos
Título:
Drosophila genome-wide RNAi screen identifies multiple regulators of HIF dependent transcription in hypoxia
Autor/es:
DEKANTY ANDRÉS; ROMERO NURIA M.; BERTOLIN AGUSTINA; MARÍA G. THOMAS; CLAUDIA LEISHMAN; JOEL I. PEREZ-PERRI; GRACIELA L. BOCCACCIO; PABLO WAPPNER
Revista:
PLOS GENETICS
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Año: 2010 vol. 6 p. 1 - 10
ISSN:
1553-7390
Resumen:
Hypoxia-inducible factors (HIFs) are a family of evolutionary conserved alpha-beta heterodimeric transcription factors that
induce a wide range of genes in response to low oxygen tension. Molecular mechanisms that mediate oxygen-dependent
HIF regulation operate at the level of the alpha subunit, controlling protein stability, subcellular localization, and
transcriptional coactivator recruitment. We have conducted an unbiased genome-wide RNA interference (RNAi) screen in
Drosophila cells aimed to the identification of genes required for HIF activity. After 3 rounds of selection, 30 genes emerged
as critical HIF regulators in hypoxia, most of which had not been previously associated with HIF biology. The list of genes
includes components of chromatin remodeling complexes, transcription elongation factors, and translational regulators.
One remarkable hit was the argonaute 1 (ago1) gene, a central element of the microRNA (miRNA) translational silencing
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
as critical HIF regulators in hypoxia, most of which had not been previously associated with HIF biology. The list of genes
includes components of chromatin remodeling complexes, transcription elongation factors, and translational regulators.
One remarkable hit was the argonaute 1 (ago1) gene, a central element of the microRNA (miRNA) translational silencing
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
as critical HIF regulators in hypoxia, most of which had not been previously associated with HIF biology. The list of genes
includes components of chromatin remodeling complexes, transcription elongation factors, and translational regulators.
One remarkable hit was the argonaute 1 (ago1) gene, a central element of the microRNA (miRNA) translational silencing
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
as critical HIF regulators in hypoxia, most of which had not been previously associated with HIF biology. The list of genes
includes components of chromatin remodeling complexes, transcription elongation factors, and translational regulators.
One remarkable hit was the argonaute 1 (ago1) gene, a central element of the microRNA (miRNA) translational silencing
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
as critical HIF regulators in hypoxia, most of which had not been previously associated with HIF biology. The list of genes
includes components of chromatin remodeling complexes, transcription elongation factors, and translational regulators.
One remarkable hit was the argonaute 1 (ago1) gene, a central element of the microRNA (miRNA) translational silencing
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
as critical HIF regulators in hypoxia, most of which had not been previously associated with HIF biology. The list of genes
includes components of chromatin remodeling complexes, transcription elongation factors, and translational regulators.
One remarkable hit was the argonaute 1 (ago1) gene, a central element of the microRNA (miRNA) translational silencing
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
cells aimed to the identification of genes required for HIF activity. After 3 rounds of selection, 30 genes emerged
as critical HIF regulators in hypoxia, most of which had not been previously associated with HIF biology. The list of genes
includes components of chromatin remodeling complexes, transcription elongation factors, and translational regulators.
One remarkable hit was the argonaute 1 (ago1) gene, a central element of the microRNA (miRNA) translational silencing
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.
argonaute 1 (ago1) gene, a central element of the microRNA (miRNA) translational silencing
machinery. Further studies confirmed the physiological role of the miRNA machinery in HIFdependent transcription. This
study reveals the occurrence of novel mechanisms of HIF regulation, which might contribute to developing novel strategies
for therapeutic intervention of HIFrelated pathologies, including heart attack, cancer, and stroke.