DMT1 iron uptake in the PNS: bridging the gap between injury and regeneration

MARTINEZ-VIVOT, R.; COPELLO, G; LEAL M.C; PIÑEIRO, G; USACH, V; ROZENSZAJN,M; MORELLI L; SETTON-AVRUJ, PC

METALLOMICS

ROYAL SOC CHEMISTRY

Lugar: CAMBRIDGE; Año: 2015

1756-5901

Previous studies by our group demonstrated the key role of iron in Schwann cell maturation through anincrease in cAMP, PKA activation and CREB phosphorylation. These studies opened the door to furtherresearch on non-transferrin-bound iron uptake, which revealed the presence of DMT1 mRNA all alongSC progeny, hinting at a constitutive role of DMT1 in ensuring the provision of iron in the PNS. In lightof these previous results, the present work evaluates the participation of DMT1 in the remyelinationprocess following a demyelinating lesion promoted by sciatic nerve crush ? a reversible model of Walleriandegeneration. DMT1 was observed to colocalize with a SC marker S100b at all survival times analyzed. Inturn, the assessment of DMT1 mRNA expression exhibited an increase 7 days post-injury, while DMT1protein levels showed an increase 14 days after crush at the lesion site and distal stump; finally, an increasein iron levels became evident as from 14 days post-injury, in parallel with DMT1 values. To sum up, thepresent work unveils the role of DMT1 in mediating the neuroregenerative action of iron