High fat diet and natriuretic peptide system in skeletal muscle

SOSA, AGUSTINA; ZAGO, VALERIA; CANIFFI, CAROLINA; SORIA, DAMIÁN; ELESGARAY, ROSANA; ARRANZ CRISTINA; TOMAT, ANALÍA; SCHREIER, LAURA

Santiago de Chile

Congreso; SCHCF + ALACF 2020 joint meeting; 2020

Sociedad Chilena de Ciencias Fisiológicas (SCHCF)

Introduction: It has been postulated that the combination of an increased fat mass and a decreased skeletal muscle (SM) mass favors the development of cardiovascular diseases. Natriuretic peptides (NP) modulate cardiovascular homeostasis but the knowledge about their role on SM is limited. The bioavailability of NP depends on the neprilysin enzyme (NEP) that is responsible for the degradation of NP, and the natriuretic peptide receptor C (NPR-C) that acts as a clearance receptor. Objective: To evaluate the expression of components responsible for the degradation of NP in SM of rats fed high-fat diet (HFD) for 11 weeks.Experimental design: Wistar rats received, from weaning until the 14th week of life, HFD (60% of fat) or standard diet (SD). At the end of the experimental period, body weight (BW) and systolic blood pressure (SBP) were measured, and oral glucose tolerance test (OGTT) was performed. Animals were sacrificed, and NEP and NPR-C expression were evaluated (RT-qPCR) in SM. The protocol was approved by Ethic Committee CICUAL-FFyB-UBA. Results are expressed as mean±SEM. Statistical analysis: Student test (n=6 rats/group; *p