UMYMFOR   05516
UNIDAD DE MICROANALISIS Y METODOS FISICOS EN QUIMICA ORGANICA
Unidad Ejecutora - UE
artículos
Título:
An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells
Autor/es:
EDUARDO M. RUSTOY, PATRICIA CERRUTTI, MIGUEL A. GALVAGNO, ALICIA BALDESSARI
Revista:
BIOCATALYSIS AND BIOTRANSFORMATION
Editorial:
Taylor & Francis
Referencias:
Lugar: Oxon, Reino Unido; Año: 2006
ISSN:
1024-2422
Resumen:
  Whole cells of the yeast Rhodotorula minuta were used in the biotransformation of dialkyl esters of 2-oxoglutaric acid. Almost 100% of conversion with 97-98% of enantiomeric excess of the (S) form of 2-hydroxydiesters was obtained through an enantioselective reduction of dimethyl and diethyl 2-oxoglutarate. When longer alkoxy chain 2-oxoglutarates were used as substrates, the corresponding 4-hydroxybutyric esters were obtained, suggesting a combination process including hydrolysis, decarboxylation and reduction. The cells showed a remarkable high productivity; high conversion and enantiomeric excess were obtained at 2 g wet weight/mmol substrate.