Effects of gonadotrophins and insulin on glucose uptake in the porcine cumulus–oocyte complex during IVM

BARRIOS EXPÓSITO, MARÍA JOSEFINA; MORADO, SERGIO; ELIA, EVELIN; CETICA, PABLO DANIEL; ALVAREZ, GABRIEL MARTÍN; PAZ, DANTE

REPRODUCTION FERTILITY AND DEVELOPMENT

CSIRO PUBLISHING

Año: 2019 vol. 31 p. 1353 - 1359

1031-3613

The combination of gonadotrophins (LH and FSH) and insulin is frequently used in porcine oocyte IVM, but the individual effects of gonadotrophins and insulin have not been completely studied. The aim of this study was to investigate the mechanisms involved in glucose metabolism in the swine cumulus-oocyte complex (COC), analysing the effects of gonadotrophins (10IUmL-1 LH+10IUmL-1 FSH) and 0.4μUmL-1insulin, during 44h of IVM, on glucose transport and consumption, as well as on nuclear maturation and sperm penetration. We evaluated the effects of gonadotrophins and insulin separately or in combination on glucose consumption, membrane permeability to the glucose fluorescent analogue 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-6-deoxyglucose (6-NBDG), the presence of GLUT-4 and oocyte maturation rates, after 44h of IVM. Nuclear maturation percentages increased significantly following the addition of gonadotrophins alone or in combination with insulin to the culture medium (P