PORPHYRINOGENIC AGENTS AFFECT MITOCHONDRIAL FUNCTION IN A MOUSE MODEL OF ACUTE INTERMITTENT PORPHYRIA

MARTINEZ MARIA DEL CARMEN; BUZALEH, ANA MARÍA; ZUCCOLI, JOHANNA ROMINA; BATLLE ALCIRA; RUSPINI, SILVINA FERNANDA

Mar del Plata

Congreso; Reunion Conjunta de Biociencias; 2019

SAIC

Mitochondria plays a vital role in energy metabolism because oxidative phosphorylation and ATP synthesis occurred inside them. Heme is a prosthetic group of several hemeproteins such as Complex II, III, IV and mitochondrial Nitric Oxide Synthase (mtNOS). Previously we demonstrated that porphyrinogenic agents affected several brain metabolisms including respiratory chain and Krebs cycle in CF1 mice and a murine genetic model of Acute intermittent Porphyria (AIP). The aim was to further investigate the effects of porphyrinogenic agents on mitochondrial metabolism in AIP mice. Complex IV, V and mtNOS expression and mitochondrial integrity were evaluated. Experiments were done in brain mitochondria of AIP male mice receiving Isoflurane (2 ml/kg), Sevoflurane (1.5 ml/kg), ethanol (30%), allylisopropylacetamide (AIA, 350 mg/kg), Veronal (167 mg/kg) or starved (24 hours). Complex IV expression was only diminished by Veronal (50%, p