CIPYP   05508
CENTRO DE INVESTIGACIONES SOBRE PORFIRINAS Y PORFIRIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Nitric oxide mediated antioxidant enzymes changes protect soybean leaveas agains UV-B
Autor/es:
GIACOMETTI R; SANTA CRUZ DIEGO; NORIEGA GUILLERMO; BALESTRASSE KARINA
Lugar:
Mendoza
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).; 2012
Institución organizadora:
SAIB
Resumen:
In soybean previous studies enabled the identification of MAPK3
and 6 whose activity is enhanced within the signaling pathway
leading to defense reactions. In this study the effects of different
compounds related to hemeoxygenase (HO-1) biosynthesis on
mitogen-activated protein kinase (MAPKs) genes expression in
soybean seeds were tested. To this end, 20µM hemine, 22µM
ZnPPIX, 0.5mM furidine or 100µM 8-bromoguanosine 3',5'-cyclic
monophosphate (8Br) were added to pre-hydrated seeds for 5 days.
MAPKs genes expression was enhanced in seeds treated with
hemine. This result indicates that heme catabolism could be
involved in the signaling mediated by this cascade pathway. To
confirm this hypothesis experiments were carried out in the
presence of ZnPPIX, a potent irreversible HO-1 inhibitor. In this
case, no gene induction was observed. On the other hand, 8Br, a
cGMP analog, induced HO-1 gene expression but did not modulate
MAPKs, indicating that this effect could not be mediated by
cGMP. When the action of furidine, an abscisic acid inhibitor, was
tested a diminution of HO-1 gene expression was observed. In this
regard, MAPKs showed a different response, being MAPK6 the
only transcript that showed a diminished respect to controls, while
MAPK3 mRNA as well as MAPKK1 was enhanced. These results
were confirmed by western blotting and activity determinations.