ANALYSIS OF HOMOTYPIC AND HETEROTYPIC SPHEROIDS CELL CULTURE CONDITIONS AS A PROMISING TOOL FOR HAIR FOLLICLE REGENERATION

ANA GABRIELA KUSINSKY; MARÍA EUGENIA BALAÑÁ; FLORENCIA MAIA OPPENHEIMER; GUSTAVO JOSÉ LEIRÓS; JULIETA CERUTI

Buenos Aires

Congreso; Reunion Conjunta de Biociencias 2017; 2017

Varias

Skin substitutes generated by tissue engineering intends to mimic the normal histological structure of skin. They are composed of a matrix component andepidermal and dermal cells. Hair follicle stem cells (HFSCs) can differentiate into epidermis, sebaceous glands, and eight different types of hair follicle epithelialcells, when combined with inductive dermal cells. Dermal papilla cells (DPCs) have shown inductive properties over these epidermal stem cells, but this inductiveability tends to be lost with successive passages in 2D culture. It is possible to produce self-assembled DPC in vitro, which maintain their regeneration potential ofhair follicles in vivo. Another option is the use of hybrid micro-tissues generated from keratinocytes and DPCs, which would allow the development of organ germssimilar to hair follicles.Wnt signaling pathway is essential in maintaining the hair-inducing activity of the dermal papilla. In the other hand, we have previously shown that, in composite skin substitutes, DPCs favors early neovascularization and contributes to the neovascular network maturation, resulting in a better healing process and graft take [Leiros et al, Stem Cells Transl Med, 2014 ].Our objectives were to test different culture conditions to: a) generate DPC spheroids to improve their inductive signatures. b) generate heterotypic spheroids containing adult human DPC and HFSC in order obtain hair follicle precursors to be used in complete skin regeneration.