ICT - MILSTEIN   05483
INSTITUTO DE CIENCIA Y TECNOLOGIA "DR. CESAR MILSTEIN"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Glycosylation profile of the recombinant BVDV glycoprotein E2 expressed in tobacco callus cultures
Autor/es:
ANABELA CUTINE; PATRICIA L. MARCONI; RABINOVICH, GABRIEL A.; MARIA ALEJANDRA ALVAREZ; MARIÑO, KARINA
Reunión:
Simposio; Primer Simposio Argentino de Glicobiologia GlycoAR; 2014
Resumen:
Bovine Viral
Diarrhea (BVD) is a widespread disease that causes serious mucosal lesions and
other clinical manifestations in cattle, with a negative economic impact. The
glycoprotein E2 is the major target of the protective immune response against
the BVD virus (BVDV) infection, and could potentially be used as a vaccine
against the disease.
We have
established tobacco recombinant in vitro
cultures that express variants of the truncated protein E2 (tE2): two recombinant lines were used, one of them
with a C-terminal protein tag (KDEL), an endoplasmic reticulum (ER) retention
signal which increases the expression level of proteins in transgenic tobacco.
Using these tobacco extracts we have confirmed that they exert a protective
immune response in guinea pigs.
As the
biological activity of many glycoproteins relies on their glycosylation
profile, and that modifications in the processing of complex glycans in the
Golgi apparatus differ between plants and mammals, we
have applied different approaches to characterize the glycan structures present
in these recombinant E2 forms. Identifying the
glycosylation pattern of the recombinant E2 glycoprotein is critical in defining its immunogenicity and might be useful for
the development of effective vaccines.