Expression of recombinant human prethrombin-2 in Nicotiana tabacum.

LAGUÍA BECHER, M; NELSON, G.; MARCONI, P.L.; ALVAREZ, M.A.

Mar del Plata

Simposio; . VIII Congreso Latinoamericano y del Caribe de Biotecnología y IX Simposio Nacional de Biotecnología, REDBIO ARGENTINA 2013; 2013

RedBio Argentina

Plants represent a safe and cost-effective option to current expression systems for large-scale production of recombinant proteins. In this study, we evaluated the expression of human prethrombin-2 (FII) in two different subcellular compartments: the apoplast (FII-A) and the endoplasmic reticulum (FII-ER). The FII gene constructs were cloned under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter with or without the 5? leader sequence Ω of Tobacco Mosaic Virus (TMV). All the FII sequences were transiently expressed in tobacco leaves by agroinfiltration. In addition, FII was co-expressed with the capsid protein from Turnip Crinkle Virus (TCV-CP), which is known to act as a suppressor of gene-silencing.