ICT - MILSTEIN   05483
INSTITUTO DE CIENCIA Y TECNOLOGIA "DR. CESAR MILSTEIN"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IMPROVEMENT OF PEPTIDE FOOT AND MOUTH DISEASE VIRUS (FMDV) VACCINE FOR CATTLE
Autor/es:
CAPOZZO, A.V.; WILDA, MAXIMILIANO; BUCAFUSCO, DANILO; LAVORIA, MARÍA DE LOS ÁNGELES; FRANCO MAHECHA, OLGA; MANSILLA, FLORENCIA; PÉREZ FILGUEIRA, D MARIANO; GRIGERA, PABLO
Lugar:
Lima
Reunión:
Congreso; InmunoPerú 2012; 2012
Institución organizadora:
Asociación Latinoamericana de Inmunología
Resumen:
Epitope-based
vaccine design has been studied as alternatives to the current Foot and
Mouth Disease Virus (FMDV) inactivated vaccine. However, most of the
neutralizing-protective antibodies are directed to the antigenic site A
(ASA), the major epitope on the VP1 capsid protein (aa 139-149, FMDV-C3
serotype), which has conformational flexibility on the viral particle,
which must be resembled by the peptide to induce neutralizing
antibodies. To overcome this limitation and add ubiquous T-cell
epitopes, we developed a chimeric antigen using Vesicular Stomatitis
Virus Glycoprotein (VSV-G) as carrier of an in tandem-dimer of ASA This
particular design exposes one ASA motif in the peptide surface. The
G-ASA construct was expressed in the Baculovirus system to produce a
recombinant protein (DEL BAC) and was also prepared as a DNA vaccine (pC
DEL). Calves vaccinated with both immunogens elicited antibodies that
recognized the ASA in whole virion and were able to neutralize FMDV
infectivity in vitro. After two vaccine doses, DEL BAC induced serum
neutralizing titers compatible with an ?Expected percentage of
protection? above 90%. Plasmid pC DEL stimulated FMDV specific humoral
responses earlier than DEL BAC, though IgG1 to IgG2 ratios were lower
than those induced by both DEL BAC and inactivated FMDV-C3 after the
second dose. DEL BAC induced FMDV-specific secretion of IFN-γ in
peripheral blood mononuclear cells of outbred cattle immunized with
commercial FMDV vaccine, suggesting its capacity to recall anamnestic
responses mediated by functional T cell epitopes. The results show that
exposing FMDV-VP1 major neutralizing antigenic site in the context of
N-terminal sequences of the VSV G protein can overcome the immunological
limitations of FMDV-VP1 peptides as effective protein and DNA vaccines
for cattle