The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from Erlenmeyer flasks to a 2-l bioreactor

MARCONI, P.L.; ALVAREZ, M.A.

BIOTECHNOLOGY PROGRESS

AMER CHEMICAL SOC

Lugar: Washington; Año: 2014 vol. 30 p. 1185 - 1189

8756-7938

The effect of two protein stabilizers (polyvinylpyrrolidone and gelatine) on growth and 14D9 yield of Nicotiana tabacum cell suspension cultures (Ab-KDEL and sec-Ab) was analysed. The addition of polyvinylpyrrolidone (PVP) at a concentration of 1.0 g l-1 PVP produced the highest total 14D9 yield (biomass + culture medium) in the Ab-KDEL line (4.82 % TSP). With the addition of gelatine, the highest total 14D9 yield (2.48 % TSP) was attained in the Ab-KDEL line at 5.0 g l-1 gelatine. When the Ab-KDEL suspended cells were cultured in a 2-l bioreactor, the highest 14D9 yield was 8.10% TSP at a 5% W/V inoculum size, which was the best 14D9 yield so far obtained in the platforms tested (E. coli, N. tabacum leaves and seeds, N. tabacum hairy roots, and cell suspension cultures).