PRODUCTION OF THE 14D9 ANTIBODY BY NICOTIANA TABACUM CELL SUSPENSION BATCH CULTURE IN A 2L-BIOREACTOR

MARCONI PATRICIA L.; PETRUCCELLI SILVANA; ALVAREZ MARIA A

PLANT CELL TISSUE AND ORGAN CULTURE

SPRINGER

Lugar: Berlin; Año: 2012 p. 1 - 14

0167-6857

We have performed the scale up of a Nicotiana tabacum cell suspension culture of the transgenic line Ab-KDEL expressing the recombinant catalytic antibody 14D9 (r14D9) from 225 ml-Erlenmeyer flasks to a 2L-bioreactor. To set up the batch processes, volumetric oxygen transfer coefficient (KLa) was adjusted to 42.7/h both in Erlenmeyer flasks and bioreactor. The influence of different inoculum sizes (1%, 5% and 10% w/v) on growth and antibody production was tested. The maximal specific growth rate (0.150/d), doubling time (5d), biomass (78.5g/l), maximal r14D9 productivity (1.31 µg r14D9/g DW.d), antibody accumulation in the biomass (157 µg r14D9/mg) and in the culture medium ( 8.7 µg r14D9/mL) were achieved at 1% (w/p) inoculum size