SAUROXINE QUANTIFICATION IN RAT BRAIN

VALLEJO, M.G.; BIRRI, M.A.; ORTEGA, M.G.; CABRAL PÉREZ, M.; CABRERA, J.L; AGNESE, A.M.

Córdoba

Simposio; X Simposio Argentino y XIII Simposio Latinoamericano de Farmacobotánica; 2010

Facultad de Ciencias Químicas, UNC-IMBIV

In previous reports we have demonstrated that the Huperzia saururus (Lam.) Trevis. alkaloids have notable effects on CNS in related to memory phenomena. On the other hand, biodistribution of a drug is an important parameter to be considered in early steps of a pharmacological effects study in view that they can vary if the concentration in the target organ is not the appropriated.  For this reason, in the present work the concentration of sauroxina (Sx) (one of the majority alkaloids) level is examined in rat brain. With this purpose, male Sprague-Dowley rats (60-70 days, 200-250 g) were injected via i.p. with a dose of  30 mg/kg of Sx chlorhidrate; 30 min later rats were decapitated, their brains dissected and homogenized in HCl 0,1 M. After centrifugation, supernatant was alkalinized and partitioned with ClCH3.  This extract was analyzed by GLC-MS. For quantification, Sx was used as external standard.  The obtained line equation allow us to determine that the Sx concentration in the homogenate was of 0,33 mg/mL, so it can be deduced that the Sx content in the brain is  7,11 µg/g, representing a 0,13% of the initial administration. These data allow us to conclude that Sx cross the blood-brain barrier (BBB). Besides, it is possible to asseverate that the percentage that cross the BBB is very low, and that this would be enough to produce a pharmacological effect. According to previous experiments, were the effect of Sx on learning and memory were studied in behavioral assays (Step-down), 1 ng/rat was injected via intra-hippocampal showing an important modification in these fenomena.