Evaluation of antioxidant activity in leaves extracts from Jodina rhombifolia

PAMELA SOLEDAD BUSTOS; MICAELA PAULA DEL GAUDIO; MARÍA GABRIELA ORTEGA

Córdoba

Congreso; III REUNIÓN INTERNACIONAL DE CIENCIAS FARMACÉUTICAS (RICIFA); 2014

Facultad de Ciencias Químicas, Universidad Nacional de Córdoba

Generation of reactive oxygen (ROS) and nitrogen (RNS) species in biological media are responsible for oxidative damage to biomolecules such as DNA, lipids and proteins, producing alterations in its structure and promoting tumoral, ulcer, and inflammatory processes and various degenerative diseases. Scientific evidence indicates that ROS and RNS are implicated in inflammatory etiopathology events. In this sense, our research group has focused the study of the biological activity of medicinal plants reputed as analgesic and anti-inflammatory and its relationship to the inhibition of ROS and RNS by extracts obtained from them. Jodina rhombifolia (Hook. et Arn.) Reissek commonly known as "sombra de toro" is one of the species studied in the group. Barks and leaves of this medicinal plant are used as anti-inflammatory airways (among other uses). Different extracts obtained from this species were studied as antiproliferative, antibacterial, antifungal and anti-inflammatory activity (assessed by producing carrageenan paw edema). Previous studies of our group, informed the presence of alkaloids and other metabolites already reported for this species (phenolic compounds, tannins, flavonoids, steroids.); and evaluated the inhibitory activity on nitric oxide production, from macrophage cell line. In the present work we evaluated the antioxidant properties of chloroformic and ethanolic extract obtained from leaves of J. rhombifolia by different in vitro tests: 1) Total antioxidant potential: phosphomolybdenum method (PM) and 2) Radical scavenging capacity by TEAC methodology (Trolox Equivalent Antioxidant Capacity). Results showed an inhibition in both methodologies by the chloroform extract, in a dose-dependent manner. Besides, the extract showed an important TEAC of 98% at 100 ug/mL, being lower in the PM. These results could indicate that the alkaloid-enriched extract is the most active as free radical scavenger. We could infer, at least in part, that the presence of antioxidant compounds could correlate with the anti-inflammatory activity evaluated. However, more chemical studies are necessary in order to indicate which is the metabolite responsible for the scavenger activity.