Novel terpenoids from Plenckia intergerrima and biotechnology of Maytenus canarensis

GUTIÉRREZ NICOLÁS FATIMA; ESTÉVEZ-BRAUN, ANA; ZÁRATE, RAFAEL; OBERTI JUAN C.; RAVELO G. ANGEL

Lanzarote, España

Congreso; 4th meeting of the Canaryan Island.Young Cancer Research Investigators; 2007

Instituto Canario de Investigación del Cáncer (ICIC)

Novel terpenoids from Plenckia integerrima and biotechnology of Maytenus canariensis F. Gutiérrez-Nicolás1,2, A. Estévez-Braun 1,2, R. Zárate 1,2, J. C. Oberti3 and A. G. Ravelo 1,2. 1 Instituto Universitario de Bio-Orgánica “Antonio González”, Universidad de La Laguna, Av. Francisco Sánchez 2, 38206 La Laguna,Tenerife, Spain. 2Instituto Canario de Investigación del Cáncer (ICIC) La Laguna,Tenerife, Spain. 3 Facultad de Ciencias Químicas, Universidad de Córdoba. Av. Haya de la Torre s/n, Córdoba, Argentina. As part of our ongoing research program to isolate bioactive compounds focused on antitumoral products belonging to the Celastraceae family used in South America1, Plenckia integerrima (Lundell), was studied. This species is used as antidysenteric and anti-rheumatic agent in popular medicine.2 The EtOH extract of the root bark of P. integerrima was repeatedly chromatographed to yield seven new terpenoids, five of them agarofuran sesquiterpenes and two known products (evonomine and rhamnose). Their structures were determined by means of 1H and 13C NMR spectroscopy studies, including 1H-13C heteronuclear correlation, (COSY, ROESY, HSQC and HMBC). All new compounds were tested on different biological assays.                                                                                                                                   Maytenus canariensis (Celastraceae) exhibits antitumoral, anti-MDR and antifeedant activities.3,4 The establishment of hairy roots (HR) cultures and vegetative multiplication has been attempted. The hairy root culture from this species is a culture system that would provide a continuous source of these valued metabolites. In this work the HR were induced with three different Agrobacterium rhizogenes strains and several culture medium were tested for establishment the HR cultures. On the other hand, a successful system for in vitro plant vegetative multiplication of this Celastraceae species, was established. Results on the phytochemical study of P. integerrima as well as results on the biotechnological approach with M. canariensis are presented. 1 Ravelo, A. G.; Estévez-Braun, A.; Chávez-Orellana, H.; Pérez-Sacau, E.; Mesa-Siverio, D. Curr. Top. Med. Chem., 4, 241 (2004). 2 Correa, M.P. Diccionario das Plantas Úteis do Brasil. Vol. V. Ministerio da Agricultura, RJ. 1969; 152. 3 Gozález, A., Jiménez, I.A, Ravelo, A.G., Sazatorli, J.G.,Bazzocchi, I.L. Tetrahedron. 1993, 49(3), 697-702. 4 González, A., Jiménez, I.A, Ravelo, A.G., Bazzocchi, I.L. Tetrahedron. 1993, 49(30), 6637-6644.