Efect of Steinernema rarum and its symbiotic bacterium Xenorhabdus szentirmaii on Nacobbus aberrans

LAX, P.; RONDAN DUEÑAS, J.; CACCIA, M.; MARRO, N.; BECERRA, A.; DOUCET, M.

La Serena

Congreso; XLV Reunión Anual de la Organización de Nematólogos de los Trópicos Americanos (ONTA).; 2013

Organización de Nematólogos de los Trópicos Americanos

EFECT OF Steinernema rarum AND ITS BACTERIUM Xenorhabdus szentirmaii ON nacobbus aberrans (Efecto de Steinernema rarum y su bacteria simbionte Xenorhabdus szentirmaii sobre Nacobbus aberrans). P. Lax1, J. Rondan Dueñas2, M. Caccia1, N. Marro2, A. Becerra2 and M.E. Doucet1. 1 IDEA (CONICET-UNC) and Centro de Zoología Aplicada, FCEFyN, UNC, Córdoba, Argentina; 2CEPROCOR, Córdoba, Argentina; 3IMBIV (CONICET-UNC), FCEFyN, UNC, Córdoba, Argentina. plax@com.uncor.edu Entomopathogenic nematodos (EPN) (families Steinernematidae and Heterorhabditidae) are used as biological control agents of insecto pests. Infective juveniles (IJ) of the nematode carry symbiotic bacteria (SB) in their instentine that kill the host. The EPN-SB complex has been show to have an antagonistic effect on certain plant-parasitic nematodes. The SB Xenorhabdus szentirmaii was isolated from an Argentine isolate of Steinernema rarum. The nematicidal activity of the SB on second stage juveniles and on hatching of Nacobbus aberrans eggs was evaluated in vitro. In addition, under controlled conditions the effect of IJ application of the EPN in the soil was analyzed, as well as the effect of the SB and its metabolites on N. Aberrans in tomato. The results showed that the number of galls and the reproduction factor of the plant-parasitic nematode decreased significantly in the treated plants, both in sterile and non-sterile soil. The results obteined show the promising potential of the NEP-SB complex for the management of N. aberrans.