Antioxidant effect of Quercetin on the response SOD induced by Ciprofloxacin

BUSTOS, PAMELA SOLEDAD; PÁEZ, PAULINA LAURA; CABRERA, JOSÉ LUIS; ALBESA, INÉS; ORTEGA, MARÍA GABRIELA

Buenos Aires

Congreso; VIII INTERNATIONAL CONGRESS OF THE SOCIETY FREE RADICAL BIOLOGY AND MEDICINE-SOUTH AMERICAN GROUP; 2013

SOCIETY FOR FREE RADICAL BIOLOGY AND MEDICINE-SOUTH AMERICAN GROUP

Some side effects of certain antibiotics, would relate with the ability to increase oxidative stress in human cells. In order to find natural compounds that neutralize the toxicity of reactive oxygen species (ROS) generated, we evaluated the effect of Quercetin (Q), a flavonoid with antioxidant and free radical scavenging properties isolated from Flaveria bidentis leaves, as potential protective agent. Previous studies carried out by our group demonstrated a marked protective effect of Q against ROS production induced by Ciprofloxacin (CIP) in human leukocytes. For these reason, the aim of this study was evaluate the effect of Q on endogenous antioxidant defenses like Superoxide Dismutase (SOD, endogenous metalloenzyme able to reduce oxidative stress by dismutation of superoxide anion) in presence of CIP. SOD activity in human leukocytes was evaluated by Riboflavin and NBT assay under three conditions: pre-incubation with Q, pre-incubation with CIP and simultaneous incubation with Q and CIP. Ciprofloxacin increased SOD´s activity at 0.015 and 0.5 mg/ml. Q at 10, 50 and 250 µM decreased SOD´s activity showing the greatest protective effect at 10 µM. The greatest decrease in SOD activity by Q was observed after pre-incubation with CIP. These results are in agreement with those obtained previously in our group, and demonstrate a marked protective activity against oxidative stress Q generated by CIP in human leukocytes.